open FASTA,"$ARGV[0]" or die "can not open GFF file,$!"; open GFF,"$ARGV[1]" or die "can not open FASTA file,$!"; while (<FASTA>) { chomp; if (/>/){$key=$_;$key =~ s/>//g;} else {$hash{$key}.=$_} }#把基因序列文件扫描进去hash表 while (<GFF>) { chomp; @F=split; next unless $F[2] eq "gene"; $out=substr($hash{$F[0]},$F[3]-1,$F[4]-1); print ">$F[1]\n$out\n"; }